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碧云天 IL17F Rabbit Pol... 当商品进行补货时,我们将以短信、邮件的形式通知您,最多发送一次,不会对您造成干扰。 |
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产品编号 | 产品名称 | 产品包装 | 产品价格 |
AF7263 | IL17F Rabbit Polyclonal Antibody | 50μl | 593.00元 |
来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
Rabbit | WB, IF | H, M | 18kDa | 18kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
1:500-1:2000 | - | 1:50-1:100 | - | - | - | - | - |
抗体详细信息如下:
About this Antibody | |
Name | IL17F Rabbit Polyclonal Antibody |
Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
Isotype | IgG |
Purification method | Affinity purification |
Positive samples | K-562, U-87MG |
Cellular location | Secreted |
Customer validation | - |
About the Immunogen | |
Immunogen | Recombinant fusion protein of human IL17F (NP_443104.1). |
Sequence | RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ |
Gene ID | 112744 |
Swiss Prot | Q96PD4 |
Synonyms | IL17F; CANDF6; IL-17F; ML-1; ML1; interleukin-17F |
Category | Cytokines |
Background | The IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE. IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells. IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides. In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases. IL-17E promotes Th2 cell responses. The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines. IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1. Unlike most cytokines that signal through Jak/STAT pathways, IL-17 signaling results in NF-κB activation. IL-17F is a cysteine-linked proinflammatory cytokine that can dimerize with itself or form heterodimers with the IL-17 family member that it shares 50% homology with, IL-17A. Although mainly produced by Th17 cells, IL-17F expression has been observed in several cell types including activated CD8+ T cells, γδ T cells, NKT cells, B cells and LTi cells. IL-17F binds to a heterodimeric receptor consisting of IL-17RA and IL-17RC, which upon binding induces the TRAF6-mediated activation of TAK and the Erk1/2 MAP kinase pathway. This induces the expression of numerous inflammatory chemokines and cytokines including IL-1β, IL-6, IL-8, and MIP-1β along with increased adhesion molecule expression in human airway epithelial cells, vein endothelial cells, and fibroblasts. IL-17F has been linked with asthma and other autoimmune diseases including rheumatoid arthritis, multiple sclerosis, psoriasis, and inflammatory bowel disease. |
包装清单:
产品编号 | 产品名称 | 包装 |
AF7263 | IL17F Rabbit Polyclonal Antibody | 50μl |
AZ050 | Western一抗稀释液 | 50ml |
— | 说明书 | 1份 |
保存条件:
IL17F Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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