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- 仓储包装
- 常见问题
碧云天 Recombinant Huma... 当商品进行补货时,我们将以短信、邮件的形式通知您,最多发送一次,不会对您造成干扰。 |
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产品编号 | 产品名称 | 产品包装 | 产品价格 |
P7239-5μg | Recombinant Human DHH C23II | 5μg | 669.00元 |
P7239-100μg | Recombinant Human DHH C23II | 100μg | 5702.00元 |
P7239-1mg | Recombinant Human DHH C23II | 1mg | 18280.00元 |
Species | Gene ID | Accession | Source | Length | MW | Tag |
Human | 50846 | O43323 | E. coli | 177aa | 19.9kDa | - |
About this Antibody | |
Name | Recombinant Human DHH C23II (Recombinant Human Desert Hedgehog Cys23IleIle; rHuDHH C23II);重组人沙漠刺猬因子C23II |
Synonyms | DHH; desert hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; HHG-3MGC35145 |
Purity | >96% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45μg/ml. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 10mM PB, pH6.0, 300mM NaCl. |
Endotoxin | Less than 1EU/μg of rHuDHH C23II as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Desert hedgehog protein (DHH), also named HHG-3, is belonging to the hedgehog (Hh) family that are highly conserved proteins which are widely represented throughout the animal kingdom. This protein is predicted to be made as a precursor that is autocatalytically cleaved and the biologically active form of Hh molecules corresponds to approximately the N-terminal one half of the precursor molecule. The N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. DHH is essential for a variety of patterning events during development as intercellular signal. It may function as a spermatocyte survival factor in the testes and play an important role in testes development. Mature human DHH shares 97% amino acid sequence identity with murine DHH. |
Amino Acid Sequence | IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG |
包装清单:
产品编号 | 产品名称 | 包装 |
P7239-5μg | Recombinant Human DHH C23II | 5μg |
P7239-25μg | Recombinant Human DHH C23II | 25μg |
P7239-100μg | Recombinant Human DHH C23II | 100μg |
P7239-1mg | Recombinant Human DHH C23II | 1mg |
- | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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